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Quantity One software is a powerful, flexible package for imaging and analyzing 1-D electrophoretic gels, dot blots, slot blots, and for colony counting. Quantity One software can quantitate and analyze a variety of data, including radioactive, chemiluminescent, fluorescent, and color-stained samples acquired from densitometers, storage phosphor imagers, fluorescent imagers, and gel documentation systems. Quantity One provides automated analysis for fast, high-quality results.



Image Acquisition

Laboratories with diverse imaging needs can increase productivity with a single imaging center for densitometry, gel documentation, and phosphor or fluorescence imaging, using Quantity One software. Integrated image analysis systems eliminate the expense and training time needed for other complex, application-specific software packages. You can connect one or more of Bio-Rad"s imaging systems to a single PC or Macintosh computer to control the scanning and analysis of all of your laboratory"s samples.


Image Optimization and Visualization

Quantity One includes tools to optimize the acquired gel image to perfection. Publication-quality images are easily generated with a comprehensive range of image optimization tools.

* Adjustment of brightness, contrast, and image filtering
* Full incremental image rotation
* Color palette for realistic color representation
* Multichannel image merging of 3 images to independent color channels for convenient viewing of multicolor merged data
* 3D Viewer for 3-D modeling of any user-defined area of the gel
* Image Stacking for aligned viewing of multiple gel images


Lane and Band Analysis

Quantity One offers comprehensive and sophisticated analysis for 1-D gels and blots.

* Automatic lane and band detection
* Rapid molecular weight determinations with choice of multiple regression models
* Band/lane matching analysis with comparative dendrogram creation
* Background subtraction correction of gradient gels
* Gaussian fitting of overlapping bands provides accurate quantitative analysis


Quantitation

Whether you are quantitating a DNA gel or analyzing protein expression, Quantity One has sophisticated volume tools for accurate results.
Accurate concentration analysis using sophisticated volume tools, volume box, volume circle, volume contour, or freehand drawing
Local background subtraction for individual bands


Colony Counting

The Colony Counting tool in Quantity One automatically counts the number of white or blue colonies or plaques in a Petri dish image.


Array Analysis

Quantity One easily creates volume arrays for dot blots, slot blots, and medium-density arrays for easy quantitation.


Annotations

Quantity One features powerful annotation tools to add text comments, arrows, and lines to your images. For publication-quality pictures, annotations can easily be configured with the desired properties (font type, size, and color) and automatically aligned to any direction.


Automation

The Automation Manager in Quantity One allows you to automate repeated analysis. Recallable templates save s such as lanes, automated band detection, standard bandsets, volume overlays, and text and line overlays in a template file.